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KMID : 1225720160080050412
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Allergy, Asthma & Immunology Research : AAIR
2016 Volume.8 No. 5 p.412 ~ p.420
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Short-, Intermediate-, and Long-Term Changes in Basophil Reactivity Induced by Venom Immunotherapy
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Ana Rodriguez Trabado
Carmen Camara Hijon
Alfonso Ramos Cantarino
Silvia Romero-Chala
Jose Antonio Garcia-Trujillo
Luis Miguel Fernandez Pereira
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Abstract
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Purpose: The basophil activation test (BAT) has been used to monitor venom immunotherapy (VIT) due to its high specificity. A previous study has reported a good correlation between a significant decrease in basophil activation during 5 years of VIT and clinical protection assessed by sting challenge. The following prospective study was performed to examine changes in basophil reactivity over a complete VIT period of 5 years.
Methods: BAT in a dose-response curve was studied prospectively in 10 hymenoptera venom-allergic patients over 5 years of VIT. BAT was performed at the time of diagnosis, 1 month after finishing the VIT build-up phase, and 3, 6, 12, 24, and 60 months after beginning treatment. The repeated measures ANOVA was applied to evaluate basophil activation changes throughout VIT. A cross-sectional study was also performed in 6 patients who received treatment for more than 3 years, and in another 12 patients who followed immunotherapy for at least 5 years.
Results: An early activation decrease was observed during the first 3 months of treatment, compared to pre-treatment values. This activation decrease was not maintained 6 to 18 months after treatment, but was observed again after 2 years of treatment, and maintained until the completion of the 5-year immunotherapy period. In cross-sectional analysis, the 6 patients who received treatment for 3 years, and 9 of the 12 patients who received treatment for 5 years, had negative BAT results. Three patients in this last group had positive BAT results and 2 patients had systemic reactions after field stings.
Conclusions: BAT appears to be an optimal non-invasive test for close monitoring of VIT.
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KEYWORD
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Activation, basophil, venom, immunotherapy, CD63
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